By Hiroyuki Mizuguchi, Takao Hayakawa (auth.), Sanetaka Shirahata, Kiichiro Teruya, Yoshinori Katakura (eds.)
Animal mobilephone know-how is a turning out to be self-discipline of mobile biology, which goals not just to appreciate buildings, services, and behaviours of differentiated animal cells but in addition to examine their skill for use for business and clinical reasons. The objective of animal cellphone expertise comprises accomplishments of clonal enlargement of differentiated cells with invaluable skill, optimisation in their tradition stipulations, modulation in their skill for construction of medically and pharmaceutically very important proteins, and the appliance of animal cells to gene remedy, synthetic organs, and useful meals. This quantity offers the reader an entire assessment of the current cutting-edge in Japan and different international locations the place this box is easily complex. The lawsuits might be valuable for phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers, and different disciplines relating to animal mobile tradition, operating in both educational environments or in industries of biotechnology and pharmacy.
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Extra resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Thirteenth Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Fukuoka-Karatsu, November 16–21, 2000
Abstract Zebrafish synthesize a conserved form of fibronectin (FNl) that possesses 12 type I, 2 type II, 17 type III repeats including two alternative splice sites (EIIIA and EIIIB) and a variable region (V). As in other vertebrate fibronectins, zebrafish FNI contains an RGD cell adhesion site in type III 10 and a second cell-binding site (LDV) in the V region. Analysis ofFN cDNAs indicate that a second form of fibronectin is produced in zebrafish (FN2) that exhibits a truncated structure possessing 9 type I, 2 type II and the first three type III repeats.
44 decreased during culturing of zebrafish embryonic cells in vitro, while vas expression remained constant and was not affected by bFGF in culture medium. Predictably, bFGF treatment increased expression of ntl, gsc and myoD. In contrast, bFGF suppressed expression of neural markers; wnt-1 and en-3 were strongly inhibited, while pax[zf-a] expression was influenced marginally. If bFGF was not added to primary cultures of zebrafish embryonic cells, time-dependent expression of all studied genes was observed, although wnt-1 and myoD expression was quickly down regulated during the period of culture in vitro.
Kluwer Academic Publishers, the Netherlands, pp. 93-96 (1998). Shirahata, S. : Telomere shortening in cancer cells by electrolyzed-reduced water In "Animal Cell Technology: Challenges for the 21"' Century", (ed. ), Kluwer Academic Publishers, the Netherlands, pp. 355-359 (1999). Shirahata, S. : Anti-oxidative Water Improves Diabetes. In: Animal Cell Technology: From Target to Market (ed. by E. ), Kluwer Academic Publishers, the Netherlands, in press. Sohal R S. and Weindruch R: Oxidative stress, caloric restriction, and aging.